The reaction occurs?in vivo?in the opposite direction. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin B, butirosin, neomycin and ribostamycin. Works in combination with EC?1.1.3.43, paromamine 6-oxidase, to replace the 6′-hydroxy group of paromamine with an amino group. The enzyme from the bacterium?Streptomyces kanamyceticus?can also catalyse EC?2.6.1.94, 2′-deamino-2′-hydroxyneamine transaminase, which leads to production of kanamycin A [3]. The enzyme from the bacterium?Streptomyces fradiae?can also catalyse EC?2.6.1.95, leading to production of neomycin C [2].
