In archaea and eukarya selenocysteine formation is achieved by a two-step process:?O-phosphoseryl-tRNASec?kinase (PSTK) phosphorylates the endogenous?L-seryl-tRNASec?to?O-phospho-L-seryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to?L-selenocysteinyl-tRNASec?by EC?2.9.1.2?(Sep-tRNA:Sec-tRNA synthase).
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Atp + L-seryl-tRNA(Sec) = adp + O-phospho-L-seryl-tRNA(Sec).