The enzyme, originally isolated from the bacterium?Bacillus halodurans?C-125, releases the xylose unit at the reducing end of oligosaccharides ending with the structure ¦Â-D-xylopyranosyl-(1¡ú4)-¦Â-D-xylopyranosyl-(1¡ú4)-¦Â-D-xylopyranose, leaving the new reducing end in the ¦Á configuration. It is specific for the ¦Â anomers of xylooligosaccharides whose degree of polymerization is equal to or greater than 3. The penultimate residue must be ¦Â-D-xylopyranose, but replacing either of the flanking residues with glucose merely slows the rate greatly.
Cofactor
History
Reactions
Hydrolysis of (1->4)-beta-D-xylose residues from the reducing end of oligosaccharides.
