The enzyme from?Escherichia coli?is a bifunctional fusion protein that also catalyses EC?1.17.1.7, 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase. Combined the two activities result in a two-step conversion of oxepin-CoA to 3-oxo-5,6-dehydrosuberyl-CoA, part of an aerobic phenylacetate degradation pathway [1,3,4]. The enzyme from?Escherichia coli?also exhibits enoyl-CoA hydratase activity utilizing crotonyl-CoA as a substrate [2].
