| Product Name |
prokaryotic ubiquitin-like protein ligase |
| Example Structure |
 |
| Synonyms |
PafA, proteasome accessory factor A, Pup ligase, Pup-protein ligase, Rv2097c, ubiquitin-like protein ligase |
| EC Number |
6.3.1.19 |
| CAS Registry Number |
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| Comments |
The enzyme has been characterized from the bacteria?Mycobacterium tuberculosis?and?Corynebacterium glutamicum. It catalyses the ligation of the prokaryotic ubiquitin-like protein (Pup) to a target protein by forming a bond between an ¦Å-amino group of a lysine residue of the target protein and the ¦Ã-carboxylate of the C-terminal glutamate of the ubiquitin-like protein (Pup). The attachment of Pup, also known as Pupylation, marks proteins for proteasomal degradation. Formerly EC?6.3.2.n2. |
| Cofactor |
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| History |
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| Reactions |
Atp + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L- lysine = Adp + phosphate + N(6)-([prokaryotic ubiquitin-like protein]- gamma-L-glutamyl)-[protein]-L-lysine. |
