Nonenzymatic glycation is an important factor in the pathogenesis of diabetic complications. Key early intermediates in this process are fructosamines, such as [protein]-N6-D-fructosyl-L-lysine. Fructosamine-3-kinase is part of an ATP-dependent system for removing carbohydrates from nonenzymatically glycated proteins. The phosphorylation destablilizes the [protein]-N6-D-fructosyl-L-lysine adduct and leads to its spontaneous decomposition. cf. EC?2.7.1.172, protein-ribulosamine 3-kinase.
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Atp + [protein]-N(6)-D-fructosyl-L-lysine = adp + [protein]-N(6)-(3-O- phospho-D-fructosyl)-L-lysine.