Rat GDF15 Protein, Accession#: Q9Z0J6, CAS#: 3202-706-1277; ChemWhat Code: 1413751
Overview
| Product Description | Recombinant Rat GDF15 Protein is expressed from E.coli with His tag at the N-terminal.It contains Ser189-Ala303. |
| Biotinylated |
Specification
| Target | GDF15 |
| Synonyms | |
| Gene/Protein ID (Accession) | Q9Z0J6 |
| Species | Rat |
| Exact Sequence | Ser189-Ala303 |
| Molecular Weight | The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Rat GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 6.2ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-His |
| Express System | E.coli |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9). |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1413751 | > 95% as determined by Tris-Bis PAGE |
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