| Official Full Name |
Recombinant Human B-Cell Maturation Antigen/TNFRSF17 (rHuBCMA/TNFRSF17) |
| Squence |
 |
| Amino Acid Sequence |
AGQCSQNEYF DSLLHACIPC QLRCSSNTPP LTCQRYCNAS VTNSVKGTNA |
| Synonyms |
TNFRSF17, BCM, CD269 |
| Accession Number |
Q02223 |
| GeneID |
608 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 5.4 kDa, a single non-glycosylated polypeptide chain containing 50 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated murine B cells is no less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 104 IU/mg in the presence of 100 ng/ml human APRIL. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA. |
| Endotoxin |
Less than 1 EU/ug of rHuBCMA as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Kim J, Gross JA, Dillon SR, et al. 2011. Autoimmunity, 44: 69-81.2. Sun J, Feng J, Li Y, et al. 2006. Biochem Biophys Res Commun, 346: 1158-62.3. Pelletier M, Thompson JS, Qian F, et al. 2003. J Biol Chem, 278: 33127-33.4. Laabi Y, Gras MP, Brouet JC, et al. 1994. Nucleic Acids Res, 22: 1147-54. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
