Official Full Name |
Recombinant Human Interferon-alpha2c, Yeast (rHuIFN-alpha2c,Yeast) |
Squence |
|
Amino Acid Sequence |
|
Synonyms |
|
Accession Number |
|
GeneID |
|
Summary |
IFN-alphas are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-alpha family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. |
Source |
Yeast |
Molecular Weight |
|
Biological Activity |
Fully biologically active when compared to standard. The activity is determined by the cytopathic effect inhibition assay. |
Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4, with 0.02 % Tween-20. |
Endotoxin |
Less than 0.1 EU/ug of rHuIFN-alpha2c, Yeast as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
References |
|
SDS-PAGE |
|
Safety Data Sheet (SDS) Download |
Click to download |
Technical Data Sheet (TDS) Download |
Click to download |