| Official Full Name |
Recombinant Human Keratinocyte Growth Factor-2/FGF-10 (rHuKGF-2/FGF-10) |
| Squence |
 |
| Amino Acid Sequence |
LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS |
| Synonyms |
|
| Accession Number |
O15520 |
| GeneID |
2255 |
| Summary |
|
| Source |
|
| Molecular Weight |
Approximately 19.1 kDa, a single, non-glycosylated polypeptide chain containing 169 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/ug of rHuKGF-2/FGF-10 as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4.2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404.3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63.4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5.5. Entesarian M, Matsson H, Klar J, et al. 2005. Nat Genet. 37:125-7. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
