| Official Full Name |
Recombinant Human Macrophage Migration Inhibitory Factor, His (rHuMIF,His) |
| Squence |
 |
| Amino Acid Sequence |
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFALEHHH HHH |
| Synonyms |
GLF, L-dopachrome Isomerase, Phenylpyruvate Tautomerase |
| Accession Number |
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| GeneID |
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| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 13.5 kDa, a single non-glycosylated polypeptide chain containing 117 amino acids, with 6 × His at the C-terminus. |
| Biological Activity |
Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA. |
| Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin |
Less than 1 EU/ug of rHuMIF, His as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Edwards KM, Tomfohr LM, Mills PJ, et al. 2011. Sleep, 34: 161-3.2. Delaloye J, De Bruin IJ, Darling KE, et al. 2012. Cytokine, 3. Leu RW, Woodson PD, Whitley SB. 1977. J Reticuloendothel Soc, 22: 329-40.4. Landolfo S. 1977. G Batteriol Virol Immunol, 70: 137-43.5. Baugh JA, Chitnis S, Donnelly SC, et al. 2002. Genes Immun, 3: 170-6. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
