Recombinant Human Platelet-derived Growth Factor-AA (rHuPDGF-AA)
Squence
Amino Acid Sequence
Synonyms
Accession Number
GeneID
Summary
Source
Escherichia coli.
Molecular Weight
Biological Activity
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 50-200 ng/mL in a fluorometric assay using the redox sensitive dye.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0.
Endotoxin
Less than 0.1 EU/ug of rHuPDGF-AA as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
