Recombinant Human soluble Tumor Necrosis Factor Receptor Type I/TNFRSF1A (rHusTNFRI/TNFRSF1A, P19438, 7132) CAS#: 103-2818-1816; ChemWhat Code: 1405225

Official Full Name Recombinant Human soluble Tumor Necrosis Factor Receptor Type I/TNFRSF1A (rHusTNFRI/TNFRSF1A)
Squence Squence of Recombinant Human soluble Tumor Necrosis Factor Receptor Type I/TNFRSF1A
Amino Acid Sequence IYPSGVIGLV PHLGDREKRD SVCPQGKYIH PQNNSICCTK CHKGTYLYND CPGPGQDTDC RECESGSFTA SENHLRHCLS CSKCRKEMGQ VEISSCTVDR DTVCGCRKNQ YRHYWSENLF QCFNCSLCLN GTVHLSCQEK QNTVCTCHAG FFLRENECVS CSNCKKSLEC TKLCLPQIEN VKGTEDSGTT
Synonyms
Accession Number P19438
GeneID 7132
Summary
Source
Molecular Weight Approximately 21.2 kDa, a single non-glycosylated polypeptide chain containing 190 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 cells is less than 0.05 ug/ml, corresponding to a specific activity of > 2 × 104IU/mg in the presence of 0.25 ng/mL of rHuTNF-alpha.
Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Endotoxin Less than 0.1 EU/ug of rHusTNF RI/TNFRSF1A as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Human soluble Tumor Necrosis Factor Receptor Type I/TNFRSF1A
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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