HR6B, Ubiquitin Carrier Protein B, Ubiquitin-protein Ligase B
Accession Number
GeneID
Summary
Source
Escherichia coli.
Molecular Weight
Approximately 19.0 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids of human UBE2B and 14 a.a. vector sequence including 6 × His tag at N-terminus.
Biological Activity
No data available.
Appearance
Sterile Colorless liquid.
Formulation
A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.6 with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.
Endotoxin
Less than 1 EU/ug of rHuUBE2B, His as determined by LAL method.
Reconstitution
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.
References
1. Roest HP, van Klaveren J, de Wit J, et al. 1996. Cell, 86: 799-810.2. Adegoke OA, Bedard N, Roest HP, et al. 2002. Am J Physiol Endocrinol Metab, 283: E482-9.3. Kavakebi P, Hausott B, Tomasino A, et al. 2005. Mol Cell Neurosci, 29: 559-68.4. Mulugeta Achame E, Wassenaar E, Hoogerbrugge JW, et al. 2010. BMC Genomics, 11: 367.
