Recombinant Murine Glial Cell Line-derived Neurotrophic Factor (rMuGDNF, P48540, 14573) CAS#: 127-14-1816; ChemWhat Code: 1405130

Official Full Name Recombinant Murine Glial Cell Line-derived Neurotrophic Factor (rMuGDNF)
Squence Squence of Recombinant Murine Glial Cell Line-derived Neurotrophic Factor
Amino Acid Sequence SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCESAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDNLVY HILRKHSAKR CGCI
Synonyms
Accession Number P48540
GeneID 14573
Summary
Source
Molecular Weight Approximately 29.9 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 0.05 % Tween-20.
Endotoxin Less than 0.1 EU/ug of rMuGDNF as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Murine Glial Cell Line-derived Neurotrophic Factor
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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