Recombinant PreScission Protease (rPPase, , ) CAS#: 461-03-1816; ChemWhat Code: 1405248

Official Full Name Recombinant PreScission Protease (rPPase)
Squence Squence of Recombinant PreScission Protease
Amino Acid Sequence
Synonyms 3C protease, Picornain 3C, PSP
Accession Number
GeneID
Summary PreScission protease is a cysteine protease derived from human rhinovirus – HRV3C Protease. rPP is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. It specifically recognizes the amino acid sequences which include the core site of Leu-Phe-Gln-Gly-Pro and cleaves between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the super structures of the fusion protein. rPP works most effective at 4°C and can digest substrates at room temperature as well.
Source Escherichia coli.
Molecular Weight PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.
Biological Activity
Appearance Sterile colorless liquid.
Formulation 50 mM Tris-HCl, pH 7.0 (at 25°C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5 °C prior to use.
Endotoxin
Reconstitution
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.
References 1. Werner G, Rosenwirth B, Bauer E, et al. 1986. J Virol, 57: 1084-93.2. Libby RT, Cosman D, Cooney MK, et al. 1988. Biochemistry, 27: 6262-8.3. Aschauer B, Werner G, McCray J, et al. 1991. Virology, 184: 587-94.4. Leong LE, Walker PA, Porter AG. 1993. J Biol Chem, 268: 25735-9.
SDS-PAGE SDS-PAGE of Recombinant PreScission Protease
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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