Official Full Name |
Recombinant Rat Eotaxin-2/CCL24 (rRtEotaxin-2/CCL24) |
Squence |
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Amino Acid Sequence |
MPTGSVTIPS SCCVTFISKK IPVNRVISYQ LANGSICPKA GVIFITKKGH KICTDPKLPW VQKHIKNLDA KRNQPSEGAK ALGPKFVIQK LRGNSTKV |
Synonyms |
CK-beta-6, MPIF-2, Small-inducible Cytokine A24 |
Accession Number |
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GeneID |
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Summary |
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Source |
Escherichia coli. |
Molecular Weight |
Approximately 10.5 kDa, a single non-glycosylated polypeptide chain consisting of an N-terminal Methionine and the mature rat Eotaxin-2. |
Biological Activity |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-250 ng/ml. |
Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4. |
Endotoxin |
Less than 1 EU/ug of rRtEotaxin-2/CCL24 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
References |
1. Owczarek W, Paplinska M, Targowski T, et al. 2010. Cytokine, 50: 181-5.2. Ben-Yehuda C, Bader R, Puxeddu I, et al. 2008. Exp Lung Res, 34: 467-79.3. Schaefer D, Meyer JE, Pods R, et al. 2006. Int Arch Allergy Immunol, 140: 205-14. |
SDS-PAGE |
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Safety Data Sheet (SDS) Download |
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Technical Data Sheet (TDS) Download |
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