Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10-15 M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.
Source
Molecular Weight
Biological Activity
Appearance
Sterile Colorless liquid.
Formulation
Supplied as a 0.2 um filtered concentrated sterile solution in 20 mM potassium dihydrogen phosphate buffer, pH 6.5.
Endotoxin
Less than 0.1 EU/ug of rStreptavidin as determined by LAL method.
Reconstitution
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 24 months from date of receipt, -20 to -70 °C as supplied.- Refer to lot specific COA for the Use by Date when stored at ≤ -20 °C as supplied.
