| Product Name |
RNA 3′-terminal-phosphate cyclase (ATP) |
| Example Structure |
 |
| Synonyms |
PH1529, Rcl1p, RNA 3′-phosphate cyclase, RNA 3′-teminal phosphate cyclase, RNA 3′-terminal phosphate cyclase, RNA 3′-terminal phosphate cylase, RNA cyclase, RNA-3′-phosphate cyclase, RNL, Rtc, Rtc1, RtcA, St-Rtc |
| EC Number |
6.5.1.4 |
| CAS Registry Number |
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| Comments |
The enzyme converts the 3′-terminal phosphate of various RNA substrates into the 2′,3′-cyclic phosphodiester in an ATP-dependent reaction. Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a histidine residue [5,6]. The adenylate group is then transferred to the 3′-phosphate terminus of the substrate, forming the capped structure [RNA]-3′-(5′-diphosphoadenosine). Finally, the enzyme catalyses an attack of the vicinal O-2′ on the 3′-phosphorus, which results in formation of cyclic phosphate and release of the adenylate. The enzyme also has a polynucleotide 5′ adenylation activity [7].?cf. EC?6.5.1.5, RNA 3′-terminal-phosphate cyclase (GTP). |
| Cofactor |
|
| History |
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| Reactions |
Atp + (RNA)-3′-(3′-phospho-ribonucleoside) = amp + diphosphate + (RNA)- 3′-(2′,3′-cyclophospho)-ribonucleoside. |
