SARS-COV-2 Spike S1 (B.1.640.2/IHU) Protein, Accession#: QHD43416, CAS#: 3202-706-392; ChemWhat Code: 1412401
Overview
| Product Description | Recombinant SARS-COV-2 Spike S1(B.1.640.2/IHU) Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gln14-Arg683(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G). |
| Biotinylated |
Specification
| Target | Spike S1 (B.1.640.2/IHU) |
| Synonyms | S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 |
| Gene/Protein ID (Accession) | QHD43416 |
| Species | SARS-COV-2 |
| Exact Sequence | Gln14-Arg683(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G) |
| Molecular Weight | The protein has a predicted MW of 74.88 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized SARS-COV-2 Spike S1 (B.1.640.2/IHU) , His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 23.0ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Endotoxin | Less than 1EU per ug by the LAL method. |
Shipping, Reconstitution & Storage
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability and Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Validation Spectrums
| Spectrums |  |
Buy Reagent | |
| ChemWhat 1412401 | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
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