toluene 2-monooxygenase EC#: 1.14.13.243; ChemWhat Code: 1375488
| Product Name | toluene 2-monooxygenase |
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| Synonyms | toluene ortho-monooxygenase, tomA1/2/3/4/5 |
| EC Number | 1.14.13.243 |
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| Comments | The enzyme, characterized from the bacterium Burkholderia cepacia, belongs to a class of nonheme, oxygen-dependent diiron enzymes. It contains a hydroxylase component with two binuclear iron centers, an NADH-oxidoreductase component containing FAD and a [2Fe-2S] iron-sulfur cluster, and a third component involved in electron transfer between the hydroxylase and the reductase. The enzyme dihydroxylates its substrate in two sequential hydroxylations, initially forming 2-methylphenol, which is hydroxylated to 3-methylcatechol. |
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