Recombinant Human Fibroblast Growth Factor 4 (rHuFGF-4, P08620, 2249) CAS#: 104-04-1816

Official Full Name Recombinant Human Fibroblast Growth Factor 4 (rHuFGF-4)
Squence Squence of Recombinant Human Fibroblast Growth Factor 4
Amino Acid Sequence GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL
Synonyms HST, HST-1, HSTF-1, HBGF-4, Transforming Protein KS3
Accession Number P08620
GeneID 2249
Summary FGF-4, also named FGF­K and K­FGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF-4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF­4 (71­206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with murine, rat, canine and bovine FGF­4. Additionally, FGF-4 shares about 30 % sequence identity with the prototypical members of the FGF family.
Source Escherichia coli.
Molecular Weight Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 1 × PBS, pH 7.4, 300 mM NaCl.
Endotoxin Less than 1 EU/ug of rHuFGF-4 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 500 mM NaCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Human Fibroblast Growth Factor 4
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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