| Official Full Name |
Recombinant Human Interferon-beta1b (rHuIFN-beta1b) |
| Squence |
 |
| Amino Acid Sequence |
MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN |
| Synonyms |
|
| Accession Number |
P01574 |
| GeneID |
3456 |
| Summary |
|
| Source |
Escherichia coli. |
| Molecular Weight |
Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids. |
| Biological Activity |
Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 3.0 × 107 IU/mg. |
| Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, containing 2 % HSA and 3 % mannitol. |
| Endotoxin |
Less than 1 EU/ug of rHuIFN-β1b as determined by LAL method. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| References |
1. Yoshino A, Tashiro S, Ogino A, et al. 2011. Int J Oncol, 39: 529-42.2. Farrell RA, Marta M, Gaeguta AJ, et al. 2012. Rheumatology (Oxford), 51: 590-9.3. Breckpot K, Corthals J, Bonehill A, et al. 2005. J Leukoc Biol, 78: 898-908.4. Oger J, Francis G, Chang P. 2005. J Neurol Sci, 237: 45-52.5. Giorelli M, Livrea P, Trojano M. 2005. J Interferon Cytokine Res, 25: 395-406. |
| SDS-PAGE |
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| Safety Data Sheet (SDS) Download |
Click to download |
| Technical Data Sheet (TDS) Download |
Click to download |
