Recombinant Human Interleukin-36 alpha, 153a.a. (rHuIL-36alpha,153a.a., Q9UHA7, 27179) CAS#: 101-362-1816; ChemWhat Code: 1404734

Official Full Name Recombinant Human Interleukin-36 alpha, 153a.a. (rHuIL-36alpha,153a.a.)
Squence Squence of Recombinant Human Interleukin-36 alpha, 153a.a.
Amino Acid Sequence KIDTPQQGSI QDINHRVWVL QDQTLIAVPR KDRMSPVTIA LISCRHVETL EKDRGNPIYL GLNGLNLCLM CAKVGDQPTL QLKEKDIMDL YNQPEPVKSF LFYHSQSGRN STFESVAFPG WFIAVSSEGG CPLILTQELG KANTTDFGLT MLF
Synonyms FIL1 epsilon, IL-1 epsilon, IL-1F6, IL-1H1
Accession Number Q9UHA7
GeneID 27179
Summary Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36β, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. Studies showed IL-36alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant human interleukin-36 alpha (153 a.a.) contains 153 amino acids residues which is a single non-glycosylated polypeptide and it shares 57 – 68 % a.a. sequence identity with mouse, rabbit, equine and bovine IL-36alpha and 27 – 57 % a.a. sequence identity with other novel IL-1 family members.
Source Escherichia coli.
Molecular Weight Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion in human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.0, 0.1 % Tween 80.
Endotoxin Less than 1 EU/ug of rHuIL-36α, 153a.a. as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Human Interleukin-36 alpha, 153a.a.
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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