Recombinant Murine Mucosae-associated Epithelial Chemokine/CCL28 (rMuMEC/CCL28, Q9JIL2, 56838) CAS#: 224-28-1816; ChemWhat Code: 1404586

Official Full Name Recombinant Murine Mucosae-associated Epithelial Chemokine/CCL28 (rMuMEC/CCL28)
Squence Squence of Recombinant Murine Mucosae-associated Epithelial Chemokine/CCL28
Amino Acid Sequence SEAILPMASS CCTEVSHHVS GRLLERVSSC SIQRADGDCD LAAVILHVKR RRICISPHNR TLKQWMRASE VKKNGRENVC SGKKQPSRKD RKGHTTRKHR TRGTHRHEAS R
Synonyms CCK1
Accession Number Q9JIL2
GeneID 56838
Summary CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC). The CCL28 mRNA is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The amino acids sequence of human and mouse CCL28 shares 83% identity, when the protein is processed to be the mature form. CCL28 is signaling through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils.
Source Escherichia coli.
Molecular Weight Approximately 12.6 kDa, a single, non-glycosylated polypeptide chain containing 111 amino acids.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration range of 1.0-10.0 ng/ml.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin Less than 1 EU/ug of rMuMEC/CCL28 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
SDS-PAGE SDS-PAGE of Recombinant Murine Mucosae-associated Epithelial Chemokine/CCL28
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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