Recombinant Rat Keratinocyte Growth Factor-2/FGF-10 (rRtKGF-2/FGF-10, P70492, 25443) CAS#: 144-10-1816

Official Full Name Recombinant Rat Keratinocyte Growth Factor-2/FGF-10 (rRtKGF-2/FGF-10)
Squence Squence of Recombinant Rat Keratinocyte Growth Factor-2/FGF-10
Accession Number P70492
GeneID 25443
Summary Fibroblast growth factor 10 is a protein which belongs to the fibroblast growth factor (FGF) family and FGF family members are involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and it plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may play a role in wound healing and is required for normal branching morphogenesis. Recombinant rat FGF-10 contains a 179 amino acids and it shares 94 % and 100 % a.a. sequence identity with human and murine FGF-10.
Source Escherichia coli.
Molecular Weight Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120 ng/ml, corresponding to a specific activity of > 8.3 × 103 IU/mg.
Appearance Sterile filtered white lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4, 5 % trehalose.
Endotoxin Less than 1 EU/ug of rRtKGF-2/FGF-10 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
SDS-PAGE SDS-PAGE of Recombinant Rat Keratinocyte Growth Factor-2/FGF-10
Safety Data Sheet (SDS) Download Click to download
Technical Data Sheet (TDS) Download Click to download

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