DNA ligase (ATP, ADP or GTP) EC#: 6.5.1.7

Product Name DNA ligase (ATP, ADP or GTP)
Example Structure Example Structure of DNA ligase (ATP, ADP or GTP) EC#: 6.5.1.7
Synonyms Hbu DNA ligase, multiple cofactor-dependent DNA ligase, Szi DNA ligase
EC Number 6.5.1.7
CAS Registry Number
Comments The enzymes from the archaea?Hyperthermus butylicus?and?Sulfophobococcus zilligii?are active with ATP, ADP or GTP. They show no activity with NAD+. The enzyme catalyses the ligation of DNA strands with 3′-hydroxyl and 5′-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in duplex DNA. Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, ADP, or GTP, forming a phosphoramide bond between adenylate/guanylate and a lysine residue. The nucleotide is then transferred to the 5′-phosphate terminus of the substrate, forming the capped structure 5′-(5′-diphosphoadenosine/guanosine)-[DNA]. Finally, the enzyme catalyses a nucleophilic attack of the 3′-OH terminus on the capped terminus, which results in formation of the phosphodiester bond and release of the nucleotide. Different from EC?6.5.1.1, DNA ligase (ATP), and EC?6.5.1.6, DNA ligase (ATP or NAD+), which cannot utilize GTP.
Cofactor
History
Reactions (1) Atp + (deoxyribonucleotide)(n)-3′-hydroxyl + 5′-phospho- (deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + Amp + diphosphate. (2) Adp + (deoxyribonucleotide)(n)-3′-hydroxyl + 5′-phospho- (deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + Amp + phosphate. (3) Gtp + (deoxyribonucleotide)(n)-3′-hydroxyl + 5′-phospho- (deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + Gmp + diphosphate.

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