Recombinant Human Osteopontin/OPN (rHuOsteopontin/OPN)
Squence
Amino Acid Sequence
Synonyms
Accession Number
GeneID
Summary
Source
Mouse myeloma cell line, NS0
Molecular Weight
Biological Activity
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 × 105 cells/well are added to a rHuOsteopontin/OPN coated plate, cell adhesion is enhanced in a dosedependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is typically 0.1-0.6 ug/mL. rHuThrombin proteolytic treatment of this rHuOsteopontin can increase HEK293 cell adhesion by about 5-fold.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from 0.2 um filtered concentrated solution in PBS, pH 7.2.
Endotoxin
Less than 0.1 EU/ug of rHuOsteopontin/OPN as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1 mg/ml. Further dilutions should be made in appropriately buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
Osteopontin (OPN), also known as SPP1, Eta-1, or BSP, is a highly acidic, 45-75 kDa secreted molecule in the SIBLING family of matricellular proteins. It can be variably modified with O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN binds several integrins and can bind additional integrins after it is proteolytically cleaved. OPN is prominently expressed in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell adhesion, and cell migration. OPN expression is upregulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and it contributes to the pathophysiology of these conditions.
