Product Name |
tRNA (N6-L-threonylcarbamoyladenosine37-C2)-methylthiotransferase |
Example Structure |
 |
Synonyms |
CDKAL1, methylthio-threonylcarbamoyl-adenosine transferase B, MtaB, YqeV |
EC Number |
2.8.4.5 |
CAS Registry Number |
|
Comments |
The enzyme, which is a member of the?S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes superfamily, binds two [4Fe-4S] clusters as well as the transferred sulfur. The sulfur donor is believed to be one of the [4Fe-4S] clusters, which is sacrificed in the process, so that?in vitro?the reaction is a single turnover. The identity of the electron donor is not known. |
Cofactor |
iron-sulfur |
History |
|
Reactions |
N(6)-L-threonylcarbamoyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio- N(6)-L-threonylcarbamoyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5′-deoxyadenosine + electron acceptor. |